Detoxification of Aflatoxin-Contaminated Maize by Neutral Electrolyzed Oxidizing Water.

Toxins (Basel). 2015 Oct 23;7(10):4294-314. doi: 10.3390/toxins7104294.

Summary

Aflatoxins, a gaggle of extraordinarily poisonous mycotoxins produced by Aspergillus flavus, A. parasiticus and A. nomius, can happen as pure contaminants of sure agricultural commodities, significantly maize. These toxins have been proven to be hepatotoxic, carcinogenic, mutagenic and trigger extreme human and animal illnesses. The effectiveness of impartial electrolyzed oxidizing water (NEW) on aflatoxin detoxing was investigated in HepG2 cells utilizing a number of validation methodologies such because the Three-(four,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay, the induction of lipid peroxidation, the oxidative injury by way of glutathione modulation, the Ames take a look at and the alkaline Comet assay. Our outcomes confirmed that, after the aflatoxin-contaminated maize containing 360 ng/g was soaked in NEW (60 mg/L obtainable chlorine, pH 7.01) throughout 15 min at room temperature, the aflatoxin content material didn’t lower as confirmed by the immunoaffinity column and extremely efficiency liquid chromatography strategies. Aflatoxin fluorescence power of detoxified samples was much like untreated samples. Nevertheless, aflatoxin-associated cytotoxicity and OPEN ACCESS Toxins 2015, 7 4295 genotoxicity results have been markedly lowered upon remedy. In keeping with these outcomes, NEW could be successfully used to detoxify aflatoxin-contaminated maize.

KEYWORDS:

aflatoxins; detoxing; maize; impartial electrolyzed oxidizing water

PMID:
26512692
PMCID:
PMC4626735
DOI:
10.3390/toxins7104294
[Indexed for MEDLINE]

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